Isolation of an Insulin-Responsive Preadipose Cell Line and a Mammary Tumor Virus-Producing, Dome-Forming Epithelial Cell Line from a Mouse Mammary Tumor. (mouse mammary tumor/mammary tumor virus/dome/preadipocyte)
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چکیده
منابع مشابه
Stimulation of mammary tumor virus production in a mouse mammary tumor cell line.
SUMMARY A mouse mammary tumor cell line (MMT), which has shown since its isolation in 1962 a limited, but continuous, budding of B particles, was stimulated to high production by alternate passages of the cells through mice or rats and tissue culture. Newborn C57BL, Af, and A mice and Amster dam/IMR rats have been used as transient hosts. Detection and semiquantitative estimations of virus prod...
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An ascites line derived from a spontaneous mouse mammary carcinoma produces, on explantation and cultivation in vitro, large amounts of oncornavirus particles. The biochemical, biophysical, and electron microscopic characteristics of the virions are described. Molecular hybridization and immunological methods identify these virions as mouse mammary tumor virus.
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A cat kidney cell line, CRFK-F2, was successfully inoculated in suspension and in monolayer culture with a purified mouse mammary tumor virus derived from RIII milk. The virus produced by the infected cells was identified by immunogluorescence, electron microscopy, and RNA-directed DNA polymerase assays; it was a B-type virion that did not cross-react with mouse or feline leukemia-sarcoma virus...
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Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and core formation. The virions showed the broad range of diameters typical of retroviruses. Betaretroviruses assemble cytoplasmically, so the broad size range cannot reflect the use of the plasma membrane as a platform for assembly.
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The morphology and production of oncornaviruses in primary cell and explant mammary adenocarcinoma cultures derived from C3Hf, BALB/cNIV, BALB/cfC3H, and C3H mice were characterized. Cultures from the four mouse strains were morphologically similar; they were all epithelial and formed hemicysts and mounds. However, the numbers of cells containing mammary tumor virus (MTV) antigens and the produ...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1984
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1984.00223.x